Intranational synergies and trade-offs reveal common and differentiated priorities of sustainable development goals in China.

Accelerating efforts for the Sustainable Development Goals requires understanding their synergies and trade-offs at the national and sub-national levels, which will help identify the key hurdles and opportunities to prioritize them in an indivisible manner for a country. Here, we present the importance of the 17 goals through synergy and trade-off networks. Our results reveal that 19 provinces show the highest trade-offs in SDG13 (Combating Climate Change) or SDG5 (Gender Equality) consistent with the national level, with other 12 provinces varying. 24 provinces show the highest synergies in SDG1 (No Poverty) or SDG6 (Clean Water and Sanitation) consistent with the national level, with the remaining 7 provinces varying. These common but differentiated SDG priorities reflect that to ensure a coordinated national response, China should pay more attention to the provincial situation, so that provincial governments can formulate more targeted policies in line with their own priorities towards accelerating sustainable development.

CircACTR2 attenuated the effects of tetramethylpyrazine on human kidney cell injury.

Tetramethylpyrazine (TMP) is one of the active ingredients of Chuan Xiong that has been reported to have effects on numerous diseases, including diabetic nephropathy (DN). Whereas, related molecular mechanisms are not fully elucidated. We aimed to explore circACTR2's role in TMP-mediated protective effects on DN. In vitro DN condition was established in human kidney cells (HK-2) by treating high glucose (HG). CCK-8 assay and flow cytometry assay were used to observe cell viability and survival. Oxidative stress was determined by the associated markers using kits. The release of inflammatory factors was detected using ELISA kits. Quantitative real-time PCR (qPCR) and western blot were utilized for expression analysis of cricACTR2, miR-140-5p, and GLI pathogenesis-related 2 (GLIPR2). The binding between miR-140-5p and circACTR2 or GLIPR2 was confirmed by dual-luciferase, RIP, and pull-down studies. HG largely induced HK-2 cell apoptosis, oxidative stress, and inflammation, which were alleviated by TMP. CircACTR2's expression was enhanced in HG-treated HK-2 cells but attenuated in HG + TMP-treated HK-2 cells. CircACTR2 overexpression attenuated the functional effects of TMP and thus restored HG-induced cell apoptosis, oxidative stress, and inflammation. CircACTR2 bound to miR-140-5p to enhance the expression of GLIPR2. MiR-140-5p restoration or GLIPR2 inhibition reversed the role of circACTR2 overexpression. CircACTR2 attenuated the protective effects of TMP on HG-induced HK-2 cell damages by regulating the miR-140-5p/GLIPR2 network, indicating that circACTR2 was involved in the functional network of TMP in DN.

Preliminary manifestation of the Yangtze River Protection Strategy in improving the carbon sink function of estuary wetlands.

In 2016, the Yangtze River Protection Strategy was proposed and a series of measures were applied to restore the health and function of the Yangtze River ecosystem. However, the impact of these measures on the carbon (C) sink capacity of the Yangtze River estuary wetlands has not been exhaustively studied. In this work, the effects of these measures on the C sink capacity of Yangtze River estuary wetlands were examined through the long-term monitoring of C fluxes, soil respiration, plant growth and water quality. The C flux of the Yangtze River estuary wetlands has become increasingly negative after the implementation of these measures, mainly owing to reduction in soil CO2 emission. The decrease in the chemical fertilizer release and returning farmland to wetland had led to the improvement of water quality in the estuary area, which further reduced soil heterotrophic microbial activity, and ultimately decreasing soil CO2 emissions of estuary wetlands.

Perturbations in gut and respiratory microbiota in COVID-19 and influenza patients: a systematic review and meta-analysis.

Objectives: Coronavirus disease-19 (COVID-19)/influenza poses unprecedented challenges to the global economy and healthcare services. Numerous studies have described alterations in the microbiome of COVID-19/influenza patients, but further investigation is needed to understand the relationship between the microbiome and these diseases. Herein, through systematic comparison between COVID-19 patients, long COVID-19 patients, influenza patients, no COVID-19/influenza controls and no COVID-19/influenza patients, we conducted a comprehensive review to describe the microbial change of respiratory tract/digestive tract in COVID-19/influenza patients. Methods: We systematically reviewed relevant literature by searching the PubMed, Embase, and Cochrane Library databases from inception to August 12, 2023. We conducted a comprehensive review to explore microbial alterations in patients with COVID-19/influenza. In addition, the data on α-diversity were summarized and analyzed by meta-analysis. Results: A total of 134 studies comparing COVID-19 patients with controls and 18 studies comparing influenza patients with controls were included. The Shannon indices of the gut and respiratory tract microbiome were slightly decreased in COVID-19/influenza patients compared to no COVID-19/influenza controls. Meanwhile, COVID-19 patients with more severe symptoms also exhibited a lower Shannon index versus COVID-19 patients with milder symptoms. The intestinal microbiome of COVID-19 patients was characterized by elevated opportunistic pathogens along with reduced short-chain fatty acid (SCFAs)-producing microbiota. Moreover, Enterobacteriaceae (including Escherichia and Enterococcus) and Lactococcus, were enriched in the gut and respiratory tract of COVID-19 patients. Conversely, Haemophilus and Neisseria showed reduced abundance in the respiratory tract of both COVID-19 and influenza patients. Conclusion: In this systematic review, we identified the microbiome in COVID-19/influenza patients in comparison with controls. The microbial changes in influenza and COVID-19 are partly similar.

The carbon budget of China: 1980-2021.

As one of the world's largest emitters of greenhouse gases, China has set itself the ambitious goal of achieving carbon peaking and carbon neutrality. Therefore, it is crucial to quantify the magnitude and trend of sources and sinks of atmospheric carbon dioxide (CO2), and to monitor China's progress toward these goals. Using state-of-the-art datasets and models, this study comprehensively estimated the anthropogenic CO2 emissions from energy, industrial processes and product use, and waste along with natural sources and sinks of CO2 for all of China during 1980-2021. To recognize the differences among various methods of estimating greenhouse emissions, the estimates are compared with China's National Greenhouse Gas Inventories (NGHGIs) for 1994, 2005, 2010, 2012, and 2014. Anthropogenic CO2 emissions in China have increased by 7.39 times from 1980 to 12.77 Gt CO2 a-1 in 2021. While benefiting from ecological projects (e.g., Three Norths Shelter Forest System Project), the land carbon sink in China has reached 1.65 Gt CO2 a-1 averaged through 2010-2021, which is almost 15.81 times that of the carbon sink in the 1980s. On average, China's terrestrial ecosystems offset 14.69% ± 2.49% of anthropogenic CO2 emissions through 2010-2021. Two provincial-level administrative regions of China, Xizang and Qinghai, have achieved carbon neutrality according to our estimates, but nearly half of the administrative regions of China have terrestrial carbon sink offsets of less than 10% of anthropogenic CO2 emissions. This study indicated a high level of consistency between NGHGIs and various datasets used for estimating fossil CO2 emissions, but found notable differences for land carbon sinks. Future estimates of the terrestrial carbon sinks of NGHGIs urgently need to be verified with process-based models which integrate the comprehensive carbon cycle processes.

Routes of Theophylline Monohydrate Dehydration Process Proposed by Mid-Frequency Raman Difference Spectra.

Two routes of the dehydration process of theophylline monohydrate have been proposed in this work from mid-frequency Raman difference spectra (MFRDS) results and experiments. MFRDS can establish short-range order correlations among various theophylline crystal forms. MFRDS results indicate that the short-range order of metastable Form III is most similar to that of monohydrate, which explains that Form III is the main dehydration products in the mild dehydration process. The phenomenon that unstable amorphous theophylline intermediate phase would appear during the dehydration process of theophylline monohydrate was confirmed indirectly by Powder X-ray Diffraction (PXRD) and optical microscope and reported in the previous reports, which could cause the nucleation of Form II, as MFRDS results indicate short-range order of amorphous solid dispersion of theophylline is most similar to that of Form II. MFRDS analysis shows the advantages in studying the phase transformation of small organic molecule crystals.

Evaporative Crystallization of Simvastatin from Different Solutions Using Mid-Frequency Raman Difference Spectra Explanations.

Amorphous simvastatin (amorphous SIM) and Form I of SIM were prepared separately from SIM acetone (AC)/ethyl acetate (ETAC)/ethanol (ET) solutions by simply controlling the solvent evaporation rate, and the kinetic formation of amorphous SIM from SIM AC/ETAC/ET solutions was explained using mid-frequency Raman difference spectra analysis. The mid-frequency Raman difference spectra analysis results indicate that the amorphous phase has close connections with solutions and might be the bridge, playing an important role in the intermediate phase, between solutions and their outcome polymorphs.

Physical and virtual nutrient flows in global telecoupled agricultural trade networks.

Global agricultural trade creates multiple telecoupled flows of nitrogen (N) and phosphorus (P). The flows of physical and virtual nutrients along with trade have discrepant effects on natural resources in different countries. However, existing literature has not quantified or analyzed such effects yet. Here we quantified the physical and virtual N and P flows embedded in the global agricultural trade networks from 1997 to 2016 and elaborated components of the telecoupling framework. The N and P flows both increased continuously and more than 25% of global consumption of nutrients in agricultural products were related to physical nutrient flows, while virtual nutrient flows were equivalent to one-third of the nutrients inputs into global agricultural system. These flows have positive telecoupling effects on saving N and P resources at the global scale. Reducing inefficient trade flows will enhance resource conservation, environmental sustainability in the hyper-globalized world.

The high organic carbon accumulation in estuarine wetlands necessarily does not represent a high CO2 sequestration capacity.

Estuarine wetlands with high organic carbon (OC) accumulation rates due to their high plant biomass and interception of tide-derived OC are generally considered as large CO2 sinks. However, our previous study found that tidal OC input seems to stimulate soil CO2 emissions, potentially weakening CO2 sequestration in estuarine wetlands. To further verify this phenomenon, we first established a structural equation model, which confirmed a positive correlation between tidal OC input and soil organic carbon (SOC) and soil respiration. We then performed trace analysis to determine the stability of SOC derived from different sources and its effect on soil CO2 emissions by analyzing the input and retention of OC derived from tides and plants in the Yangtze Estuary wetlands. From upstream to downstream, as tidal OC input decreased, the relative retention ratio of the tidal OC in wetland soil increased from 1.259 to 2.148, whereas the relative retention ratio of plant OC in the soil decreased from 61.5% to 14.8%. Our findings indicated that the degradability of tidal OC was higher upstream than that downstream, but both inhibited plant OC degradation, thus providing an important reason for the higher CO2 emissions upstream of wetlands (with higher tidal OC input). In addition, the primarily contributor to CO2 (δ13) emissions' transforming from plant SOC (81.35%) to tidal SOC (91.18%) was an increase in organic matter input from the tide in a microcosm system. Consequently, a higher CO2 output than CO2 input (plant OC) due to the ready degradation of tidal OC consequently weakens the CO2 sequestration capacity of the estuarine wetlands. This phenomenon is cause for concern regarding the CO2 sink function of estuarine wetlands intercepting large amounts of organic matter.

Higher serum adrenomedullin concentration is associated with an increased risk of gestational diabetes mellitus: A nested case-control study in Wuhan, China.

Adrenomedullin (ADM) is thought to play a significant role in regulating insulin secretion and glucose metabolism. However, studies on the relationship between ADM and gestational diabetes mellitus (GDM) are limited. We hypothesized that a higher serum ADM concentration would be associated with an increased risk of GDM. Therefore, a nested case-control study of 65 GDM cases and 130 prepregnancy body mass index, age, parity, and gestational age of blood collection-matched controls was conducted to prospectively evaluate the association between circulating ADM concentrations in early pregnancy and the risk of GDM in pregnant women based on the Tongji Birth Cohort. Serum ADM concentrations in the GDM group were higher than those in the control group (2125.04 ± 644.97 vs 1880.76 ± 581.13 pg/mL) (P = .008). Serum ADM concentration was positively associated with the risk of developing GDM (Ptrend < .05). The adjusted odds ratio (OR) comparing the highest tertile of ADM with the lowest was 2.74 (95% CI, 1.17-6.43). The risk of GDM increased by 49% (OR, 1.49; 95% CI, 1.05-2.12) for each SD increment of serum ADM. Moreover, serum ADM concentration was positively correlated with circulating total cholesterol (r = 0.204), triglycerides (r = 0.197), and systolic blood pressure (r = 0.173), but negatively correlated with circulating high-density lipoprotein cholesterol concentration (r = -0.176). Pregnant women with higher serum ADM concentrations have a markedly increased risk of developing GDM. Further studies are warranted to explore the possible thresholds of ADM that increase the risk of GDM and to confirm and elucidate the underlying mechanisms.

Climatic and biotic factors influencing regional declines and recovery of tropical forest biomass from the 2015/16 El Niño.

The 2015/16 El Niño brought severe drought and record-breaking temperatures in the tropics. Here, using satellite-based L-band microwave vegetation optical depth, we mapped changes of above-ground biomass (AGB) during the drought and in subsequent years up to 2019. Over more than 60% of drought-affected intact forests, AGB reduced during the drought, except in the wettest part of the central Amazon, where it declined 1 y later. By the end of 2019, only 40% of AGB reduced intact forests had fully recovered to the predrought level. Using random-forest models, we found that the magnitude of AGB losses during the drought was mainly associated with regionally distinct patterns of soil water deficits and soil clay content. For the AGB recovery, we found strong influences of AGB losses during the drought and of [Formula: see text]. [Formula: see text] is a parameter related to canopy structure and is defined as the ratio of two relative height (RH) metrics of Geoscience Laser Altimeter System (GLAS) waveform data-RH25 (25% energy return height) and RH100 (100% energy return height; i.e., top canopy height). A high [Formula: see text] may reflect forests with a tall understory, thick and closed canopy, and/or without degradation. Such forests with a high [Formula: see text] ([Formula: see text] ≥ 0.3) appear to have a stronger capacity to recover than low-[Formula: see text] ones. Our results highlight the importance of forest structure when predicting the consequences of future drought stress in the tropics.

Moderate Freshwater Fish Intake, but Not n-3 Polyunsaturated Fatty Acids, Is Associated with a Reduced Risk of Small for Gestational Age in a Prospective Cohort of Chinese Pregnant Women.

BACKGROUND: Although previous studies have found that maternal fish intake is associated with fetal growth, the role of freshwater fish intake remains unknown. OBJECTIVE: Our aim was to examine the relationships of freshwater fish and n-3 polyunsaturated fatty acids (PUFAs) intake with the risk of small for gestational age (SGA) in Chinese pregnant women. DESIGN: This was a prospective analysis of data from the Tongji Birth cohort in Wuhan, China, from 2018 to 2021. PARTICIPANTS/SETTINGS: This study included 1,701 pregnant women who had completed a food frequency questionnaire dietary assessment during mid-pregnancy. MAIN OUTCOME MEASURES: Intake of fish was assessed by a semi-quantitative food frequency questionnaire. Total intake of n-3 PUFAs was the sum of data collected from both dietary and supplemental sources of n-3 PUFAs. Birth information was extracted from medical records. STATISTICAL ANALYSES: Multivariate logistic regression models were applied to estimate odds ratios and 95% CIs. RESULTS: The median (interquartile range) intake of freshwater fish and total n-3 PUFAs was 12.1 (4.3 to 26.4) g/d and 68.2 (24.5 to 370.0) mg/d, respectively. Moderate intake of freshwater fish was associated with reduced risk of SGA. Compared with the lowest quintile (0-3.2 g/d), the multivariable-adjusted odds ratio for women in the fourth quintile of freshwater fish intake (17.9 to 30.0 g/d) was 0.50 (95% CI 0.25 to 0.96). We found a nonlinear association between freshwater fish intake and SGA risk (Pnonlinearity = .027). However, maternal n-3 PUFAs intake was not significantly associated with SGA risk, either from total intake or from dietary sources alone. CONCLUSIONS: Moderate freshwater fish intake during pregnancy is associated with lower risk of SGA in a Chinese population. This finding provides supportive evidence for freshwater fish intake during pregnancy, particularly for the inland areas of developing countries.

Estimating ecological sustainability in the Guangdong-Hong Kong-Macao Greater Bay Area, China: Retrospective analysis and prospective trajectories.

In recent decades, rapid urbanization and intensified global climate change have resulted in a significant difference of environment and resources distribution on space, which would cause trouble for accurate assessment of regional ecological sustainable development, especially in the large urban agglomerations. The parameters used in previous assessment methods have normally ignored spatial heterogeneity, leading to deviations in the evaluation accuracies against the context above. By incorporating remote sensing technology, this study proposed an improved emergy ecological footprint (EEF) method and a novel ecological sustainability index to comprehensively analyze the variability of ecological security states (ESS) from 1994 to 2018 in the Guangdong-Hong Kong-Macao Greater Bay Area (GBA) and to predict its sustainable growth potential based on a combined factorial decomposition and scenario analysis. Results showed that the pixel-based emergy analysis revealed significant heterogeneity over time and space under the impact of climate change and intense land use activities during the study period. The emergy carrying capacity per capita (ecc) and the emergy ecological footprint per capita (eef) also showed a significant difference between the nine cities in the GBA. In addition, the traditional EEF method, which does not consider the spatiotemporal variation, has indeed overestimated the GBA's ecc by 15% compared with our results. The ESS of the GBA gradually worsened from slight insecurity in the 1990s to moderate insecurity in 2018. If the current trends in socio-economic activities and climate change continue according to the RCP8.5 scenario in the IPCC, the ESS of the GBA will reach the extreme insecurity state in 2050. However, our scenarios show that industrial structure adjustment, energy structure optimization, and especially biological resource conservation can reduce the EFI by approximately 6.52%, 23.4%, and 30.6%, respectively. Consequently, effective implementation of the above measures can limit the increase both in emergy ecological deficit and emergy ecological footprint intensity (EFI) and, together, contribute to a higher security status in the GBA in 2050.

Tropical tall forests are more sensitive and vulnerable to drought than short forests.

Our limited understanding of the impacts of drought on tropical forests significantly impedes our ability in accurately predicting the impacts of climate change on this biome. Here, we investigated the impact of drought on the dynamics of forest canopies with different heights using time-series records of remotely sensed Ku-band vegetation optical depth (Ku-VOD), a proxy of top-canopy foliar mass and water content, and separated the signal of Ku-VOD changes into drought-induced reductions and subsequent non-drought gains. Both drought-induced reductions and non-drought increases in Ku-VOD varied significantly with canopy height. Taller tropical forests experienced greater relative Ku-VOD reductions during drought and larger non-drought increases than shorter forests, but the net effect of drought was more negative in the taller forests. Meta-analysis of in situ hydraulic traits supports the hypothesis that taller tropical forests are more vulnerable to drought stress due to smaller xylem-transport safety margins. Additionally, Ku-VOD of taller forests showed larger reductions due to increased atmospheric dryness, as assessed by vapor pressure deficit, and showed larger gains in response to enhanced water supply than shorter forests. Including the height-dependent variation of hydraulic transport in ecosystem models will improve the simulated response of tropical forests to drought.

Epidemiological and clinical features of SARS-CoV-2 cluster infection in Anhui Province, Eastern China.

BACKGROUND: COVID-19 has spread worldwide and become a pandemic. We report the epidemiological and clinical characteristics of cluster infections. METHODS: Data of clustered cases were retrieved from the public health emergency monitoring information system of China. We analyzed the incubation period, generation gap, secondary attack rate, and viral load in various grouped cases. RESULTS: A total of 60 COVID-19 infection clusters including 226 patients and 19 asymptomatic cases involving four generations were analyzed. With the increase of transmission generations, secondary attack rate decreased (P＜0.001) and severity alleviated (P = 0.008). The median incubation period and intergenerational interval were 9 and 6 days, respectively. The secondary attack rate was 7.1% in the index cases, 5.0% in the first generation, 1.0% in the second generation, and 4.7% overall. Severe cases were seen more in the index (13, 65%) and first generation (7, 35%) ones, who had a significantly higher viral load than the mild and moderate ones. CONCLUSIONS: With the increase of transmission generation, secondary infection rate and severity decreased. Severe patients had a higher virus load. Patients in the incubation period and asymptomatic carriers were potential infection sources who might play an important role in transmission.

A comprehensive framework for seasonal controls of leaf abscission and productivity in evergreen broadleaved tropical and subtropical forests.

Relationships among productivity, leaf phenology, and seasonal variation in moisture and light availability are poorly understood for evergreen broadleaved tropical/subtropical forests, which contribute 25% of terrestrial productivity. On the one hand, as moisture availability declines, trees shed leaves to reduce transpiration and the risk of hydraulic failure. On the other hand, increases in light availability promote the replacement of senescent leaves to increase productivity. Here, we provide a comprehensive framework that relates the seasonality of climate, leaf abscission, and leaf productivity across the evergreen broadleaved tropical/subtropical forest biome. The seasonal correlation between rainfall and light availability varies from strongly negative to strongly positive across the tropics and maps onto the seasonal correlation between litterfall mass and productivity for 68 forests. Where rainfall and light covary positively, litterfall and productivity also covary positively and are always greater in the wetter sunnier season. Where rainfall and light covary negatively, litterfall and productivity are always greater in the drier and sunnier season if moisture supplies remain adequate; otherwise productivity is smaller in the drier sunnier season. This framework will improve the representation of tropical/subtropical forests in Earth system models and suggests how phenology and productivity will change as climate change alters the seasonality of cloud cover and rainfall across tropical/subtropical forests.

Higher plant photosynthetic capability in autumn responding to low atmospheric vapor pressure deficit.

It has been long established that the terrestrial vegetation in spring has stronger photosynthetic capability than in autumn. However, this study challenges this consensus by comparing photosynthetic capability of terrestrial vegetation between the spring and autumn seasons based on measurements of 100 in situ eddy covariance towers over global extratropical ecosystems. At the majority of these sites, photosynthetic capability, indicated by light use efficiency (LUE) and apparent quantum efficiency, is significantly higher in autumn than in spring, due to lower atmosphere vapor pressure deficit (VPD) at the same air temperature. Seasonal VPD differences also substantially explain the interannual variability of the differences in photosynthetic capability between spring and autumn. We further reveal that VPD in autumn is significantly lower than in spring over 74.14% of extratropical areas, based on a global climate dataset. In contrast, LUE derived from a data-driven vegetation production dataset is significantly higher in autumn in over 61.02% of extratropical vegetated areas. Six Earth system models consistently projected continuous larger VPD values in spring compared with autumn, which implies that the impacts on vegetation growth will long exist and should be adequately considered when assessing the seasonal responses of terrestrial ecosystems to future climate conditions.

Spatial-Temporal Footprints Assessment and Driving Mechanism of China Household Diet Based on CHNS.

Food consumption is closely associated with resource consumption and environmental sustainability. An unreasonable dietary pattern would cause great pressure or damage to resources and the environment. It is particularly important to reduce the negative impact of household food consumption on resources and the environment while simultaneously ensuring people's nutrient intake and health. This study applied the China Health and Nutrition Survey (CHNS) database to quantitatively study the spatial-temporal analysis of multiple footprints of household food consumption at multiple scales and explored the driving mechanism of the multiple footprints. The results showed that, except land footprint (LF), the other four types of footprints all decreased at varying degrees; the water footprint (WF), carbon footprint (CF), nitrogen footprint (NF) and energy footprint (EF) decreased by 18.24%, 17.82%, 12.03% and 20.36%, respectively, from 2000 to 2011; multiple footprints of food consumption of household in Guizhou was the highest among the 12 provinces involved in the study; this shows that resource consumption (water, energy and land resource) and environmental influences (CO2 emissions and nitrogen emissions) brought by food consumption of per household in Guizhou are much greater than in other provinces, which has a negative influence on sustainable development; by analyzing the driving factors of multiple footprints, it is shown that nutrient intake, household attributes, educational level and health conditions were significantly correlated to multiple footprints. Among them, nutrient intake has greater impact on the multiple footprints of Chinese household food consumption. By comparing multiple footprints of different dietary patterns, it was found that the current Chinese dietary pattern would cause excessive resource consumption, which would bring more pressure on resources and the environment. Adjusting household living habits would possibly reverse the unsustainable situation, such as reducing the consumption of animal-derived foods and adjusting the dietary pattern of households with a higher educational level and income status. Chinese Dietary Guidelines 2016 has better sustainability; the promotion of this dietary pattern across the country would help China to relieve the pressure on resources and environment from the consumer side, promoting the realization of sustainable development.

Zinc, selenium and chromium co-supplementation improves insulin resistance by preventing hepatic endoplasmic reticulum stress in diet-induced gestational diabetes rats.

Gestational diabetes mellitus (GDM) is one of the most common pregnancy complications and results in adverse outcomes for pregnant women and their offspring. Endoplasmic reticulum (ER) stress is associated with insulin resistance and implicates in the development of GDM. Zinc, selenium and chromium have been shown to maintain glucose homeostasis via multiple mechanisms, but how these trace elements affect the insulin resistance and ER stress in GDM are largely unknown. To address this, a GDM rat model was induced by feeding female Sprague-Dawley rats a high-fat (45%) and sucrose diet, while zinc (10 mg/kg.bw), selenium (20 ug/kg.bw), chromium (20 ug/kg.bw) were daily supplemented alone or in combination from 6 weeks before mating to the end of lactation period. Maternal metabolic parameters, hepatic ER stress and insulin signaling were analyzed. The results showed that zinc, selenium and chromium co-supplementation dramatically alleviated high-fat and sucrose-induced glucose intolerance and oxidative stress during entire experiment period. Hepatic ER stress as well as the unfolded protein response was activated in GDM dams, characterized by the up-regulation of glucose-regulated protein 78, phosphorylated the protein kinase RNA-like endoplasmic reticulum kinase, and the inositol-requiring enzyme 1α. Zinc, selenium and chromium supplementation significantly prevented this activation, by which contributes to the promotion of the phosphorylated protein kinase B related insulin signaling and maintenance of glucose homeostasis. In conclusion, zinc, selenium and chromium supplementation may be a promising way to prevent the development of GDM by alleviating hepatic ER stress.